Evaluation of differential and selective media for isolation of Aeromonas and Plesiomonas spp. from human feces.
نویسندگان
چکیده
We studied nine solid and two liquid media for their suitability to select Aeromonas and Plesiomonas spp. from human stools, using artificially contaminated samples as well as 254 samples from outpatients with and without diarrhea. Media with optimal sensitivity and specificity for Aeromonas spp. were alkaline peptone-water, Trypticase soy broth with ampicillin, inositol-brilliant green-bile salts agar, dextrin-fuchsin-sulfite agar, xylose-sodium desoxycholate-citrate agar, and Pril-xylose-ampicillin agar. For Plesiomonas sp., alkaline peptone-water and inositol-brilliant green-bile salts agar were optimal. Four strains of Aeromonas spp. were detected in patient samples with these media.
منابع مشابه
Aeromonas spp as a potential cause of diarrhoea in children.
AIMS To determine the prevalence of Aeromonas spp in the faeces of children and the association with symptoms of gastroenteritis. METHODS Faecal specimens (n = 1026) were cultured for Aeromonas spp using three selective media and an enrichment broth at both 30 degrees C and 37 degrees C. The isolation of Aeromonas spp was correlated with symptoms of gastroenteritis, previous antibiotic use, a...
متن کاملIsolation and identification of Campylobacter spp. and Campylobacter coli from poultry carcasses by conventional culture method and multiplex PCR in Mashhad, Iran
The genus Campylobacter is of great importance to public health because it includes several species that may cause diarrhoea. Poultry and poultry products are known as important sources of human campylobacteriosis. In this study, during the autumn months of 2005, a total of 100 samples from poultry carcasses, representing 20 broiler flocks were obtained by rinse test, after the chilling stage ...
متن کاملIsolation, identification and antimicrobial susceptibility of pathogenic Aeromonas media isolated from diseased Koi carp (Cyprinus carpio koi)
Aeromonas infections are the most common bacterial disease of cultured fish in China. In this study, a gram-negative bacillus was isolated from the liver of diseased koi carp (Cyprinus carpio koi), and named strain KC-2. The results of morphological and biochemical tests, as well as phylogenetic analysis derived from 16S rRNA and gyrB sequences indicated that the isolated strain KC-2 was highly...
متن کاملIsolation, identification and antimicrobial susceptibility of pathogenic Aeromonas media isolated from diseased Koi carp (Cyprinus carpio koi)
Aeromonas infections are the most common bacterial disease of cultured fish in China. In this study, a gram-negative bacillus was isolated from the liver of diseased koi carp (Cyprinus carpio koi), and named strain KC-2. The results of morphological and biochemical tests, as well as phylogenetic analysis derived from 16S rRNA and gyrB sequences indicated that the isolated strain KC-2 was highly...
متن کاملبررسی انواع محیطهای غنی کننده، انتخابی و افتراقی درجداسازی و شناسایی سالمونلا در مبتلایان به اسهال
Background and Aim: Salmonellosis is a gastroenteritis caused by different serotypes of Salmonella and is the most common type of food poisoning in the world. The purpose of this research study is to optimize the conventional method for the isolation of Salmonella SPP from the diarrheic specimens of children. Materials and Methods: Stool specimens were obtained from one hundred patients admitt...
متن کاملذخیره در منابع من
با ذخیره ی این منبع در منابع من، دسترسی به آن را برای استفاده های بعدی آسان تر کنید
برای دانلود متن کامل این مقاله و بیش از 32 میلیون مقاله دیگر ابتدا ثبت نام کنید
ثبت ناماگر عضو سایت هستید لطفا وارد حساب کاربری خود شوید
ورودعنوان ژورنال:
- Journal of clinical microbiology
دوره 17 1 شماره
صفحات -
تاریخ انتشار 1983